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验证数据展示

  • WB analysis of mouse thymus using 20681-1-AP

    WB analysis of mouse thymus using 20681-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20681-1-AP

    IF Staining of HepG2 using 20681-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20681-1-AP (ABCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 20681-1-AP

    IHC staining of mouse brain using 20681-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20681-1-AP (ABCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCA2 Polyclonal antibody

ABCA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20681-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABC transporter ABCA2, ABC2, ABCA2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse thymus tissue, HepG2 cells, L02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20681-1-AP targets ABCA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family A (ABC1), member 2
Synonyms ABC transporter ABCA2, ABC2, ABCA2
Calculated Molecular Weight 270 kDa
Observed Molecular Weight 270 kDa
GenBank Accession NumberNM_001606
Gene Symbol ABCA2
Gene ID (NCBI) 20
RRIDAB_10859780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCA2, also named as ABC2 and KIAA1062, belongs to the ABC transporter ABCA2, a member of the ATP-binding cassette (ABC) transporter family, is primarily expressed in brain and is involved in brain sterol homeostasis and associated with early onset Alzheimer's disease. ABCA2 is mainly localized in endosomes and lysosomes of oligodendrocytes and Schwann cells. It has also be reported as a marker of neural progenitor in the adult rodent brain. (PMID: 16539677)

实验方案

Product Specific Protocols
WB protocol for ABCA2 antibody 20681-1-APDownload protocol
IHC protocol for ABCA2 antibody 20681-1-APDownload protocol
IF protocol for ABCA2 antibody 20681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols