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ABCB6 Polyclonal antibody

ABCB6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14996-1-AP

Print datasheet

Synonyms

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

验证数据展示

  • WB analysis of mouse brain using 14996-1-AP

    WB analysis of mouse brain using 14996-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14996-1-AP

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14996-1-AP

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14996-1-AP targets ABCB6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ABCB6 fusion protein Ag6982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 6
Synonyms ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC000559
Gene Symbol ABCB6
Gene ID (NCBI) 10058
RRIDAB_2220828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP58
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCB6 antibody 14996-1-APDownload protocol
IHC protocol for ABCB6 antibody 14996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols