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ACSM3 Polyclonal antibody

ACSM3 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10168-2-AP

Print datasheet

Synonyms

ACSM3, Butyrate CoA ligase 3, Protein SA homolog, SA, SAH



经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10168-2-AP targets ACSM3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ACSM3 fusion protein Ag0224 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA synthetase medium-chain family member 3
Synonyms ACSM3, Butyrate CoA ligase 3, Protein SA homolog, SA, SAH
Calculated Molecular Weight 49 kDa, 66 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC002790
Gene Symbol ACSM3
Gene ID (NCBI) 6296
RRIDAB_2222699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53FZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.

实验方案

Product Specific Protocols
WB protocol for ACSM3 antibody 10168-2-APDownload protocol
IHC protocol for ACSM3 antibody 10168-2-APDownload protocol
IF protocol for ACSM3 antibody 10168-2-APDownload protocol
FC protocol for ACSM3 antibody 10168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIHC

Cell

Organoid Models of Human and Mouse Ductal Pancreatic Cancer.

Authors - Sylvia F Boj
humanIHC

Front Cell Dev Biol

Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.

Authors - Lingyun Zhang
humanWB, IHC

Front Oncol

The Overexpression of Acyl-CoA Medium-Chain Synthetase-3 (ACSM3) Suppresses the Ovarian Cancer Progression via the Inhibition of Integrin β1/AKT Signaling Pathway.

Authors - Limei Yan
mouse

Sci Rep

Thioesterase induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation.

Authors - Giovan N Cholico
mouseWB,IF

Eur J Cell Biol

Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy.

Authors - Paul Dowling
humanWB

Exp Ther Med

ACSM3 suppresses proliferation and induces apoptosis and cell cycle arrest in acute myeloid leukemia cells via the regulation of IGF2BP2

Authors - Xin Zheng