smooth muscle actin specific Polyclonal antibody
smooth muscle actin specific Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
259
验证数据展示
经过测试的应用
Positive WB detected in | mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung |
Positive IHC detected in | mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse heart tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:3000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55135-1-AP targets smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | actin, alpha 2, smooth muscle, aorta |
Synonyms | a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 Smooth muscle Actin Specific, Actin, aortic smooth muscle, Alpha actin 2, Alpha SMA, Alpha SMA Specific, a-SMA, smooth muscle actin, smooth muscle actin-specific, α SMA, α-sma |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | NM_001613 |
Gene Symbol | Alpha smooth muscle actin |
Gene ID (NCBI) | 59 |
RRID | AB_10949628 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62736 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for smooth muscle actin specific antibody 55135-1-AP | Download protocol |
IHC protocol for smooth muscle actin specific antibody 55135-1-AP | Download protocol |
IF protocol for smooth muscle actin specific antibody 55135-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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