smooth muscle actin specific Polyclonal antibody

smooth muscle actin specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55135-1-AP

Print datasheet

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 Smooth muscle Actin Specific, Actin, aortic smooth muscle, Alpha actin 2, Alpha SMA, Alpha SMA Specific, a-SMA, smooth muscle actin, smooth muscle actin-specific, α SMA, α-sma



经过测试的应用

Positive WB detected inmouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung
Positive IHC detected inmouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55135-1-AP targets smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, alpha 2, smooth muscle, aorta
Synonyms a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 Smooth muscle Actin Specific, Actin, aortic smooth muscle, Alpha actin 2, Alpha SMA, Alpha SMA Specific, a-SMA, smooth muscle actin, smooth muscle actin-specific, α SMA, α-sma
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001613
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_10949628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

实验方案

Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 55135-1-APDownload protocol
IHC protocol for smooth muscle actin specific antibody 55135-1-APDownload protocol
IF protocol for smooth muscle actin specific antibody 55135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

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mouseIF

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humanIHC

Cancer Commun (Lond)

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mouseIF

Nat Commun

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humanIF

Mol Cancer

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Authors - Jing Kong
mouseWB,IF

J Nanobiotechnology

Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy

Authors - Zhenxian Li