ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18897-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

EC:1.1.1.1, ADH3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inrat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADH1C Ready-To-Use IHC Kit. ADH1C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ADH1C fusion protein Ag13528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Synonyms EC:1.1.1.1, ADH3
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 37-45 kDa
GenBank Accession NumberBC062476
Gene Symbol ADH1C
Gene ID (NCBI) 126
RRIDAB_10596950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00326
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

实验方案

Product Specific Protocols
WB protocol for ADH1C antibody 18897-1-APDownload protocol
IHC protocol for ADH1C antibody 18897-1-APDownload protocol
IF protocol for ADH1C antibody 18897-1-APDownload protocol
IP protocol for ADH1C antibody 18897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen
human,mouseWB

Biochem Pharmacol

The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis.

Authors - Feifeng Song
mouseWB

Biochem Pharmacol

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Authors - Yunhui Xu
humanWB

J Transl Med

Comprehensive analysis of the metabolomics and transcriptomics uncovers the dysregulated network and potential biomarkers of Triple Negative Breast Cancer

Authors - Sisi Gong
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