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验证数据展示

  • WB analysis of Jurkat using 66884-1-Ig

    WB analysis of Jurkat using 66884-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66884-1-Ig

    IF Staining of HepG2 using 66884-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1A antibody (66884-1-Ig, Clone: 2D4A6 ) at dilution of 1:500 and sms3AF647-1, CL488-Phalloidin (green).

ASF1A Monoclonal antibody

ASF1A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66884-1-Ig

产品说明书

CloneNo. 2D4A6

宿主/亚型

Mouse / IgG3

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

CGI-98, CGI 98, CCG1-interacting factor A, CCG1 interacting factor A, Anti-silencing function protein 1 homolog A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66884-1-Ig targets ASF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 ASF1A fusion protein Ag1233 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
别名 CGI-98, CGI 98, CCG1-interacting factor A, CCG1 interacting factor A, Anti-silencing function protein 1 homolog A
计算分子量 204 aa, 23 kDa
观测分子量 21-23 kDa
GenBank蛋白编号BC010878
基因名称 ASF1A
Gene ID (NCBI) 25842
RRIDAB_2882216
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

实验方案

Product Specific Protocols
WB protocol for ASF1A antibody 66884-1-IgDownload protocol
IF protocol for ASF1A antibody 66884-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols