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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated AURKA Monoclonal antibody

AURKA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F4B10

Cat no : CL594-66757

Print datasheet

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

验证数据展示

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66757-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66757 targets AURKA in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AURKA fusion protein Ag20096 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name aurora kinase A
Synonyms AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
RRIDAB_2883624
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

实验方案

Product Specific Protocols
IF protocol for CL594 AURKA antibody CL594-66757Download protocol
Standard Protocols
Click here to view our Standard Protocols