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BBS1 Polyclonal antibody

BBS1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 21118-1-AP

Print datasheet

Synonyms

Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2

验证数据展示

  • IF Staining of hTERT-RPE1 cells using 21118-1-AP

    IF Staining of hTERT-RPE1 cells using 21118-1-AP

    IF result (base of ciliary stain) of anti-BBS1 (21118-1-AP, 1:50) with with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

  • WB analysis of mouse kidney using 21118-1-AP

    WB analysis of mouse kidney using 21118-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21118-1-AP

    IP experiment of mouse brain using 21118-1-AP

    IP result of anti-BBS1 (IP:21118-1-AP, 4ug; Detection:21118-1-AP 1:600) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse brain tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IF detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21118-1-AP is BBS1 Fusion Protein expressed in E. coli.

Tested Applications IF, IP, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen BBS1 fusion protein Ag15460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Bardet-Biedl syndrome 1
Synonyms Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2
Calculated Molecular Weight 593 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC047642
Gene Symbol BBS1
Gene ID (NCBI) 582
RRIDAB_2878814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BBS1 antibody 21118-1-APDownload protocol
IP protocol for BBS1 antibody 21118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
View Article
humanIF

J Biol Chem

Non-redundant roles of DIAPHs in primary ciliogenesis.

Authors - Oliva Palander
View Article