验证数据展示
经过测试的应用
| Positive WB detected in | pig bone marrow tissue, Caco-2 cells, HeLa cells, pig aorta tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66383-1-Ig targets BMP2 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, Cell treatment |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 | BMP2 fusion protein Ag13501 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | bone morphogenetic protein 2 |
| 别名 | BMP 2, BMP2A, BMP-2, BMP-2A, BMP 2A |
| 计算分子量 | 396 aa, 44 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC069214 |
| 基因名称 | BMP2 |
| Gene ID (NCBI) | 650 |
| RRID | AB_2881759 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P12643 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| IHC protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Multifunctionally disordered TiO2 nanoneedles prevent periprosthetic infection and enhance osteointegration by killing bacteria and modulating the osteoimmune microenvironment | ||
ACS Appl Mater Interfaces Nitrogen-Doped Multiwalled Carbon Nanotubes Enhance Bone Remodeling through Immunomodulatory Functions. | ||
Angiogenesis Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8. | ||
Br J Pharmacol Andrographolide regulates H3 histone lactylation by interfering with p300 to alleviate aortic valve calcification | ||
Phytother Res Bavachinin selectively modulates PPAR γ and maintains bone homeostasis in Type 2 Diabetes |
