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- KD/KO Validated
ATG14/Barkor (N-terminal) Polyclonal antibody
ATG14/Barkor (N-terminal) Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
IF/ICC, IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19491-1-AP targets ATG14/Barkor (N-terminal) in WB,IHC,IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB,IP,IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, canine, bovine |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | KIAA0831 |
| Synonyms | ATG14, Atg14L, Barkor, KIAA0831 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 65-68 kDa |
| GenBank Accession Number | NM_014924 |
| Gene Symbol | KIAA0831 |
| Gene ID (NCBI) | 22863 |
| RRID | AB_10642701 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZNE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP | Download protocol |
| IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP | Download protocol |
| IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Autophagy STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. | ||
Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. | ||
Autophagy Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells. |
