• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using CL594-66020

    IF Staining of HeLa using CL594-66020

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66020 (Bcl-XL antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-66020

    FC experiment of HeLa using CL594-66020

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Bcl-XL (CL594-66020, Clone:4C12A6) (red), or 0.4 ug . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66020

产品说明书

CloneNo. 4C12A6

宿主/亚型

Mouse / IgG2b

种属反应性

human, canine

应用

IF/ICC, FC (Intra)

别名

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells, MDCK cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66020-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66020 targets Bcl-XL in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, canine
免疫原 Bcl-XL fusion protein Ag18037 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2-like 1
别名 Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092
计算分子量 26 kDa
GenBank蛋白编号BC019307
基因名称 Bcl-XL
Gene ID (NCBI) 598
RRIDAB_2883480
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07817
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

实验方案

Product Specific Protocols
IF protocol for CL594 Bcl-XL antibody CL594-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols