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验证数据展示

  • WB analysis of human plasma using 15837-1-AP

    WB analysis of human plasma using 15837-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human liver using 15837-1-AP

    IHC staining of human liver using 15837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15837-1-AP (C4BPB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 15837-1-AP

    IHC staining of human liver using 15837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15837-1-AP (C4BPB Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 15837-1-AP

    IF Staining of HepG2 using 15837-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15837-1-AP (C4BPB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

C4BPB Polyclonal antibody

C4BPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15837-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C4BP, C4b-binding protein beta chain, C4b binding protein beta chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15837-1-AP targets C4BPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C4BPB fusion protein Ag8587 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 4 binding protein, beta
Synonyms C4BP, C4b-binding protein beta chain, C4b binding protein beta chain
Calculated Molecular Weight 251 aa, 28 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC005378
Gene Symbol C4BPB
Gene ID (NCBI) 725
RRIDAB_2067035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20851
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP beta chain (C4BPB).

实验方案

Product Specific Protocols
WB protocol for C4BPB antibody 15837-1-APDownload protocol
IHC protocol for C4BPB antibody 15837-1-APDownload protocol
IF protocol for C4BPB antibody 15837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

BMC Cancer

The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.

Authors - Ping Zhao
View Article
humanWB

World J Surg Oncol

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

Authors - Wei Dong
View Article