验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, pig stomach tissue, pig brain tissue |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CA9 Ready-To-Use IHC Kit. CA9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66243-1-Ig targets CA9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, pig, rabbit |
| 文献引用反应性 | human, pig |
| 免疫原 | CA9 fusion protein Ag1540 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | carbonic anhydrase IX |
| 别名 | CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250 |
| 计算分子量 | 459 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC014950 |
| 基因名称 | CA9 |
| Gene ID (NCBI) | 768 |
| RRID | AB_2881632 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is present both on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CA9 antibody 66243-1-Ig | Download protocol |
| IHC protocol for CA9 antibody 66243-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol Identification and validation of a combined hypoxia and immune index for triple-negative breast cancer. | ||
Elife Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression | ||
Cell Rep ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis | ||
Int J Gen Med Identification of Hypoxia-Associated Signature in Colon Cancer to Assess Tumor Immune Microenvironment and Predict Prognosis Based on 14 Hypoxia-Associated Genes | ||
Mol Carcinog Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition |
