验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11533-1-AP targets CaMKII Beta in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, zebra finches |
| 免疫原 | CaMKII Beta fusion protein Ag2119 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium/calmodulin-dependent protein kinase II beta |
| 别名 | CAMK2B, CAMK2, CaMK II subunit beta, CAM2, CaM kinase II subunit beta |
| 计算分子量 | 503 aa, 56 kDa |
| 观测分子量 | 50-70 kDa |
| GenBank蛋白编号 | BC019070 |
| 基因名称 | CaMKII beta |
| Gene ID (NCBI) | 816 |
| RRID | AB_2275067 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13554 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CaMKII Beta antibody 11533-1-AP | Download protocol |
| IHC protocol for CaMKII Beta antibody 11533-1-AP | Download protocol |
| IP protocol for CaMKII Beta antibody 11533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway. | ||
Sci Total Environ Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons. | ||
Front Oncol Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.
| ||
Front Cell Neurosci Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis. | ||
Neural Plast Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model. |
