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  • KD/KO Validated

Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (6)

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2B9H8

Cat No : 66093-1-Ig

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Synonyms

Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66093-1-Ig is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, ELISA, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken, sheep, monkey, pig
Immunogen Caspase 8/p43/p18 fusion protein Ag20524 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caspase 8, apoptosis-related cysteine peptidase
Synonyms Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5
Calculated Molecular Weight 538 aa, 62 kDa
Observed Molecular Weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank Accession NumberBC028223
Gene Symbol CASP8
Gene ID (NCBI) 841
RRIDAB_11232214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

实验方案

Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IHC protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IF protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
IP protocol for Caspase 8/p43/p18 antibody 66093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.

Authors - Dianrong Li
  • KO Validated
humanWB,IHC

Acta Biomater

TRAIL-modified, doxorubicin-embedded periodic mesoporous organosilica nanoparticles for targeted drug delivery and efficient antitumor immunotherapy.

Authors - Xinyao Feng
mouseWB

J Pineal Res

Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.

Authors - Wenkai Ren
humanWB,IF

Chem Sci

Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.

Authors - Meng Liu
chickenWB

J Hazard Mater

The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells.

Authors - Shuo Zhang
humanWB

Cell Rep

Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation

Authors - Zehe Ge