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CD2 Polyclonal antibody

CD2 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10299-1-AP

Print datasheet

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

验证数据展示

  • WB analysis of Jurkat using 10299-1-AP

    WB analysis of Jurkat using 10299-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10299-1-AP (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 10299-1-AP

    IHC staining of human tonsillitis using 10299-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • IHC staining of human tonsillitis using 10299-1-AP

    IHC staining of human tonsillitis using 10299-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • FC experiment of Jurkat using 10299-1-AP

    FC experiment of Jurkat using 10299-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug CD2 antibody (10299-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10299-1-AP targets CD2 in WB, IHC, FC,ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB,ELISA
Tested Reactivity human
Immunogen CD2 fusion protein Ag0423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD2 molecule
Synonyms CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_2228874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
WB protocol for CD2 antibody 10299-1-APDownload protocol
IHC protocol for CD2 antibody 10299-1-APDownload protocol
FC protocol for CD2 antibody 10299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols