验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human spleen tissue, mouse spleen tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12837-2-AP targets CD247 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CD247 fusion protein Ag3889 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD247 molecule |
| Synonyms | T cell receptor T3 zeta chain, CD3Z, CD3Q, CD3H, CD3 ZETA |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC025703 |
| Gene Symbol | CD247 |
| Gene ID (NCBI) | 919 |
| RRID | AB_2244279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD247 antibody 12837-2-AP | Download protocol |
| IHC protocol for CD247 antibody 12837-2-AP | Download protocol |
| IF protocol for CD247 antibody 12837-2-AP | Download protocol |
| IP protocol for CD247 antibody 12837-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol The Transferrin Receptor-Directed CAR for the Therapy of Hematologic Malignancies. | ||
PLoS One Liver Sinusoidal Endothelial Cell-Mediated CD8 T Cell Priming Depends on Co-Inhibitory Signal Integration over Time. | ||
Front Immunol Distinct immune and inflammatory response patterns contribute to the identification of poor prognosis and advanced clinical characters in bladder cancer patients | ||
Cytotherapy Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma | ||
Cell Rep Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance | ||
Nat Commun Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function |
