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CoraLite® Plus 488 Anti-Human CD38 (HB7)

CD38 Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HB7

Cat no : CL488-65107

Print datasheet

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL488-65107

    FC experiment of human peripheral blood lymphocytes using CL488-65107

    100 ul human peripheral blood were surface stained 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) (red) or isotype control antibody (blue). Lymphocytes were gated for analysis. Cells were not fixed.

  • IF Staining of human peripheral blood mononuclear cells using CL488-65107

    IF Staining of human peripheral blood mononuclear cells using CL488-65107

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD38 Ready-To-Use IHC Kit. CD38 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65107-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65107 targets CD38 in IF, FC applications and shows reactivity with Human samples.

Tested Applications IF, FC Application Description
Tested Reactivity Human
Immunogen Human BJAB B cell line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD38 molecule
Synonyms ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10
Calculated Molecular Weight 300 aa, 34 kDa
GenBank Accession NumberBC007964
Gene Symbol CD38
Gene ID (NCBI) 952
ENSEMBL Gene IDENSG00000004468
RRIDAB_2883181
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD38 antibody CL488-65107Download protocol
FC protocol for CL Plus 488 CD38 antibody CL488-65107Download protocol
Standard Protocols
Click here to view our Standard Protocols