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验证数据展示

  • FC experiment of human PBMCs using PE-65143

    FC experiment of human PBMCs using PE-65143

    1X10^6 human PBMCs were surface co-stained with Atlantic Blue™ Anti-Human CD3 and 5 ul PE Anti-Human CD4 (PE-65143, Clone: RPA-T4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using PE-65143

    FC experiment of human PBMCs using PE-65143

    1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD4 (PE-65143, Clone: RPA-T4), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

PE Anti-Human CD4 (RPA-T4)

CD4 Monoclonal Antibody for FC
Cat No. PE-65143

产品说明书

CloneNo. RPA-T4

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

CD4, CD4 molecule, CD4mut

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65143 targets CD4 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD4 molecule
别名 CD4, CD4 molecule, CD4mut
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2883925
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP01730
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for PE CD4 antibody PE-65143Download protocol
Standard Protocols
Click here to view our Standard Protocols