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CD63 Polyclonal antibody

CD63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 25682-1-AP

Print datasheet

Synonyms

Ocular melanoma-associated antigen, Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen



经过测试的应用

Positive WB detected inhuman urine exosomes tissue, human serum exosomes
Positive IHC detected inhuman malignant melanoma tissue, human tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman malignant melanoma tissue
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25682-1-AP targets CD63 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, pig, rabbit, canine, monkey, chicken, zebrafish
Immunogen CD63 fusion protein Ag19690 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD63 molecule
Synonyms Ocular melanoma-associated antigen, Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberBC002349
Gene Symbol CD63
Gene ID (NCBI) 967
RRIDAB_2783831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

实验方案

Product Specific Protocols
WB protocol for CD63 antibody 25682-1-APDownload protocol
IHC protocol for CD63 antibody 25682-1-APDownload protocol
IF protocol for CD63 antibody 25682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
humanWB

Adv Sci (Weinh)

Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury

Authors - Xun Wu
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
humanWB

Biosens Bioelectron

Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis

Authors - Zhichao Fan
humanWB

Small Methods

Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers.

Authors - Xuehui Liu