CD86 Polyclonal antibody
CD86 Polyclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, IF, WB, ELISA and More (1)
Conjugate
Unconjugated
132
验证数据展示
经过测试的应用
Positive WB detected in | Daudi cells, Raji cells, Ramos cells, RAW 264.7 cells |
Positive IF detected in | raji cells |
Positive FC detected in | Raji cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13395-1-AP targets CD86 in WB, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IF, WB, ELISA |
Cited Applications | Cell treatment, FC, IF, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | CD86 fusion protein Ag4063 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD86 molecule |
Synonyms | Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, FUN 1, LAB72 |
Calculated Molecular Weight | 329 aa, 38 kDa |
Observed Molecular Weight | 60-80 kDa |
GenBank Accession Number | BC040261 |
Gene Symbol | CD86 |
Gene ID (NCBI) | 942 |
ENSEMBL Gene ID | ENSG00000114013 |
RRID | AB_2074882 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P42081 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD86 antibody 13395-1-AP | Download protocol |
IF protocol for CD86 antibody 13395-1-AP | Download protocol |
FC protocol for CD86 antibody 13395-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
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Acta Biomater Bacterial navigation for tumor targeting and photothermally-triggered bacterial ghost transformation for spatiotemporal drug release. | ||
ACS Appl Mater Interfaces Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy. |