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CDC42 Polyclonal antibody

CDC42 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10155-1-AP

Print datasheet

Synonyms

CDC42, CDC42Hs, G25K, G25K GTP binding protein



经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells, C6 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10155-1-AP targets CDC42 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, hamster, bovine
Immunogen CDC42 fusion protein Ag0207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 42 (GTP binding protein, 25kDa)
Synonyms CDC42, CDC42Hs, G25K, G25K GTP binding protein
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC002711
Gene Symbol CDC42
Gene ID (NCBI) 998
RRIDAB_2078096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60953
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.

实验方案

Product Specific Protocols
WB protocol for CDC42 antibody 10155-1-APDownload protocol
IHC protocol for CDC42 antibody 10155-1-APDownload protocol
IF protocol for CDC42 antibody 10155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

Nat Commun

Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.

Authors - Claire M Mulvey
mouseWB

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
humanWB

Nucleic Acids Res

DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Authors - Ming Gao
humanWB

Biomaterials

Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.

Authors - Teng Zhou
mouseIHC

Mol Ther Nucleic Acids

miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.

Authors - Lihui Zhang
mouseWB

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu