验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells |
Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
IF | See 2 publications below |
产品信息
20648-1-AP targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | E-cadherin |
Calculated Molecular Weight | 97 kDa |
Observed Molecular Weight | 100-125 kDa |
GenBank Accession Number | NM_004360 |
Gene Symbol | E-cadherin |
Gene ID (NCBI) | 999 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. This antibody is specific to CDH1.
实验方案
Product Specific Protocols | |
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WB protocol for E-cadherin antibody 20648-1-AP | Download protocol |
IHC protocol for E-cadherin antibody 20648-1-AP | Download protocol |
IF protocol for E-cadherin antibody 20648-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Lett SDF-1/CXCR4 Promotes epithelial-mesenchymal transition and progression of colorectal cancer by activation of the wnt/β-catenin signaling pathway. | ||
Onco Targets Ther Bioinformatics Analyses Indicate That Cathepsin G (CTSG) is a Potential Immune-Related Biomarker in Oral Squamous Cell Carcinoma (OSCC). | ||
Endocrine Adipogenic changes of hepatocytes in a high-fat diet-induced fatty liver mice model and non-alcoholic fatty liver disease patients. | ||
Tissue Cell Slc44a2 deletion alters tetraspanin and N-cadherin expression: Reduced adhesion and enhanced proliferation in cultured mesenchymal lung cells. | ||
Mol Med Rep MicroRNA-590 is an EMT-suppressive microRNA involved in the TGFβ signaling pathway. | ||
Int J Mol Sci Androgen-Responsive Oncogenic lncRNA RP11-1023L17.1 Enhances c-Myc Protein Stability in Prostate Cancer |