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  • Featured Product
  • KD/KO Validated

CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10122-1-AP

Print datasheet

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

验证数据展示

  • IHC staining of human liver cancer using 10122-1-AP

    IHC staining of human liver cancer using 10122-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK2 antibody included
  • WB analysis using 10122-1-AP

    WB analysis using 10122-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10122-1-AP

    WB analysis using 10122-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10122-1-AP

    IP experiment of HeLa using 10122-1-AP

    IP result of anti-CDK2 (IP:10122-1-AP, 4ug; Detection:10122-1-AP 1:10000) with HeLa cells lysate 1200 ug.

  • IHC staining of mouse testis using 10122-1-AP

    IHC staining of mouse testis using 10122-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK2 antibody included
  • IHC staining of mouse testis using 10122-1-AP

    IHC staining of mouse testis using 10122-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK2 antibody included
  • IHC staining of human liver cancer using 10122-1-AP

    IHC staining of human liver cancer using 10122-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK2 antibody included
  • IF Staining of mouse testis using 10122-1-AP

    IF Staining of mouse testis using 10122-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CDK2 antibody (10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 10122-1-AP

    FC experiment of HepG2 using 10122-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (10122-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using 10122-1-AP

    IF Staining of HepG2 using 10122-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (10122-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue, HEK-293 cells, Hela cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10122-1-AP targets CDK2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, sheep, fish, sea cucumbers
Immunogen CDK2 fusion protein Ag0166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 2
Synonyms p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
RRIDAB_2078556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CDK2 antibody 10122-1-APDownload protocol
IHC protocol for CDK2 antibody 10122-1-APDownload protocol
IF protocol for CDK2 antibody 10122-1-APDownload protocol
IP protocol for CDK2 antibody 10122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Signal Transduct Target Ther

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Authors - Saisai Guo
  • KD Validated
View Article
humanWB,IHC

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
  • KD Validated
View Article
humanWB

Sci Adv

Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer

Authors - Atish Mohanty
View Article
humanWB

Nat Commun

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

Authors - Xiaoyu Ma
View Article
humanWB

J Clin Invest

TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.

Authors - Zheng Hu
View Article
humanWB

Nat Commun

Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.

Authors - Jun Lu
View Article