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  • Featured Product
  • KD/KO Validated

CDK4 Polyclonal antibody

CDK4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 11026-1-AP

Print datasheet

Synonyms

PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3

验证数据展示

  • WB analysis using 11026-1-AP

    WB analysis using 11026-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11026-1-AP

    IHC staining of human lung cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human lung cancer using 11026-1-AP

    IHC staining of human lung cancer using 11026-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK4 antibody included
  • FC experiment of MCF-7 using 11026-1-AP

    FC experiment of MCF-7 using 11026-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDK4 Polyclonal antibody (11026-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 11026-1-AP

    WB analysis of HeLa using 11026-1-AP

    WB result of CDK4 antibody (11026-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK4 transfected HeLa cells.

  • IP experiment of HeLa using 11026-1-AP

    IP experiment of HeLa using 11026-1-AP

    IP result of anti-CDK4 (IP:11026-1-AP, 3ug; Detection:11026-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of MCF-7 using 11026-1-AP

    IF Staining of MCF-7 using 11026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (11026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, rat pancreas tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11026-1-AP targets CDK4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP, Cell treatment
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, zebrafish, sheep
Immunogen CDK4 fusion protein Ag1505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 4
Synonyms PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight30-34 kDa
GenBank Accession NumberBC010153
Gene Symbol CDK4
Gene ID (NCBI) 1019
RRIDAB_2078702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).

实验方案

Product Specific Protocols
WB protocol for CDK4 antibody 11026-1-APDownload protocol
IHC protocol for CDK4 antibody 11026-1-APDownload protocol
IF protocol for CDK4 antibody 11026-1-APDownload protocol
IP protocol for CDK4 antibody 11026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
View Article
humanIHC,WB

Mol Cancer

The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC.

Authors - Xiaosong Wang
View Article
humanWB

Sci Adv

The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis

Authors - Zhenlong Yu
View Article
humanWB

Nat Commun

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Authors - Youzhou Sang
View Article
humanWB

Nat Commun

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Authors - Dan Su
View Article
humanWB

J Pineal Res

Mitochondrial cytochrome P450 (CYP) 1B1 is responsible for melatonin-induced apoptosis in neural cancer cells.

Authors - Zhenlong Yu
View Article