验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, COLO 320 cells, Jurkat cells, THP-1 cells, ROS1728 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, K-562 cells, HSC-T6 cells, C6 cells, Raw 264.7 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66278-1-Ig targets CDK6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CDK6 fusion protein Ag5600 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase 6 |
| 别名 | EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6 |
| 计算分子量 | 326 aa, 36 kDa |
| 观测分子量 | 36-40 kDa |
| GenBank蛋白编号 | BC027989 |
| 基因名称 | CDK6 |
| Gene ID (NCBI) | 1021 |
| RRID | AB_2881661 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q00534 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK6 antibody 66278-1-Ig | Download protocol |
| IHC protocol for CDK6 antibody 66278-1-Ig | Download protocol |
| IF protocol for CDK6 antibody 66278-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Transcriptomics and molecular docking reveal the potential mechanism of lycorine against pancreatic cancer | ||
Int J Mol Sci (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells. | ||
Cell Oncol (Dordr) LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway. | ||
Oxid Med Cell Longev MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation | ||
Int J Oncol GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer. | ||
J Cancer Apolipoprotein L3 inhibits breast cancer proliferation and modulates cell cycle via the P53 pathway |
