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  • Featured Product
  • KD/KO Validated

CHAF1B Polyclonal antibody

CHAF1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27633-1-AP

Print datasheet

Synonyms

MPP 7, p60 CAF1, CAF1, CAF IP60, CAF I p60

验证数据展示

  • WB analysis using 27633-1-AP

    WB analysis using 27633-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27633-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27633-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27633-1-AP (CHAF1B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHAF1B antibody included
  • IHC staining of human ovary cancer using 27633-1-AP

    IHC staining of human ovary cancer using 27633-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27633-1-AP (CHAF1B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHAF1B antibody included

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHAF1B Ready-To-Use IHC Kit. CHAF1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27633-1-AP targets CHAF1B in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, ChIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen CHAF1B fusion protein Ag26504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromatin assembly factor 1, subunit B (p60)
Synonyms MPP 7, p60 CAF1, CAF1, CAF IP60, CAF I p60
Calculated Molecular Weight 559 aa, 61 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC021218
Gene Symbol CHAF1B
Gene ID (NCBI) 8208
RRIDAB_2880933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for CHAF1B antibody 27633-1-APDownload protocol
IHC protocol for CHAF1B antibody 27633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Authors - Shuai Huang
  • KD Validated
View Article
humanChIP

Cancer Res Commun

Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells

Authors - Diana Saleiro
View Article