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  • KD/KO Validated

CHRNA3 Polyclonal antibody

CHRNA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10333-1-AP

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Synonyms

CHRNA3, LNCR2, NACHRA3, PAOD2



经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, HepG2 cells, mouse thymus tissue, HEK-293 cells
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10333-1-AP targets CHRNA3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CHRNA3 fusion protein Ag0272 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 3
Synonyms CHRNA3, LNCR2, NACHRA3, PAOD2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000513
Gene Symbol CHRNA3
Gene ID (NCBI) 1136
RRIDAB_2080511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.

实验方案

Product Specific Protocols
WB protocol for CHRNA3 antibody 10333-1-APDownload protocol
IHC protocol for CHRNA3 antibody 10333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

Authors - Strunnikova N V NV
humanWB

J Invest Dermatol

Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.

Authors - Jing Wang
mouseIHC

FASEB J

The gut-brain axis rewired: adding a functional vagal nicotinic "sensory synapse"

Authors - Azucena Perez-Burgos
humanFC

Mar Drugs

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Authors - Zhihua Sun
humanWB

PLoS One

Nicotine Activates YAP1 through nAChRs Mediated Signaling in Esophageal Squamous Cell Cancer (ESCC).

Authors - Yue Zhao
  • KD Validated
humanWB

Biochem Biophys Res Commun

Nicotine induces fibrogenic changes in human liver via nicotinic acetylcholine receptors expressed on hepatic stellate cells.

Authors - Soeda Junpei J