验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
15891-1-AP targets CKM/CKB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | CKM/CKB fusion protein Ag8678 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | creatine kinase, muscle |
| 别名 | CKM, CKM/CKB, CKMM, Creatine Kinase, Creatine Kinase MM, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK |
| 计算分子量 | 381 aa, 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC007462 |
| 基因名称 | CKM |
| Gene ID (NCBI) | 1158 |
| RRID | AB_2260647 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06732 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CKM/CKB antibody 15891-1-AP | Download protocol |
| IHC protocol for CKM/CKB antibody 15891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Lipids Health Dis Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats. | ||
Spinal Cord The relationship between skin ultrasound images and muscle damage using skin blotting in wheelchair basketball athletes. | ||
Nanomedicine Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer. |
