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Collagen Type I Polyclonal antibody

Collagen Type I Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (7)

Applications

WB, IHC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14695-1-AP

Print datasheet

Synonyms

COL1A2, COL1, collagen, type I, alpha 2, Collagen alpha 2(I) chain, col I

验证数据展示

  • WB analysis of pig skin using 14695-1-AP

    WB analysis of pig skin using 14695-1-AP

    pig skin tissue were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 14695-1-AP

    IHC staining of human skin cancer using 14695-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 14695-1-AP

    IHC staining of human skin cancer using 14695-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14695-1-AP

    WB analysis using 14695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14695-1-AP

    WB analysis using 14695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14695-1-AP

    WB analysis using 14695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of U-87 MG using 14695-1-AP

    IP experiment of U-87 MG using 14695-1-AP

    IP result of anti-Collagen Type I (IP:14695-1-AP, 4ug; Detection:14695-1-AP 1:2000) with U-87 MG cells lysate 1920 ug.

  • IHC staining of human colon using 14695-1-AP

    IHC staining of human colon using 14695-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 14695-1-AP

    IHC staining of human colon using 14695-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of SW480 using 14695-1-AP

    FC experiment of SW480 using 14695-1-AP

    1X10^6 SW480 cells were intracellularly stained with 0.2 ug Anti-Human Collagen Type I (14695-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HSC-T6 using 14695-1-AP

    FC experiment of HSC-T6 using 14695-1-AP

    1X10^6 HSC-T6 cells were intracellularly stained with 0.8 ug Anti-Human Collagen Type I (14695-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inpig skin tissue, human placenta tissue, MG-63 cells, Saos-2 cells, U-87 MG cells
Positive IP detected inU-87 MG cells
Positive IHC detected inhuman skin cancer tissue, human colon tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSW480 cells, HSC-T6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14695-1-AP targets Collagen Type I in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, FC (Intra), IP, CoIP
Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, chicken, bovine, toad
Immunogen Collagen Type I fusion protein Ag6281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type I, alpha 2
Synonyms COL1A2, COL1, collagen, type I, alpha 2, Collagen alpha 2(I) chain, col I
Calculated Molecular Weight 1366 aa, 130 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC054498
Gene Symbol COL1A2
Gene ID (NCBI) 1278
RRIDAB_2082037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08123
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

实验方案

Product Specific Protocols
WB protocol for Collagen Type I antibody 14695-1-APDownload protocol
IHC protocol for Collagen Type I antibody 14695-1-APDownload protocol
IP protocol for Collagen Type I antibody 14695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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