验证数据展示
经过测试的应用
Positive WB detected in | rat skin tissue, pig skin tissue, mouse tail tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66761-1-Ig targets Collagen Type I in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | Human, pig, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, bovine |
Immunogen | Collagen Type I fusion protein Ag6281 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | collagen, type I, alpha 2 |
Synonyms | Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4 |
Calculated Molecular Weight | 1366 aa, 130 kDa |
Observed Molecular Weight | 140 kDa, 70-80 kDa |
GenBank Accession Number | BC054498 |
Gene Symbol | COL1A2 |
Gene ID (NCBI) | 1278 |
RRID | AB_2882107 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08123 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.
实验方案
Product Specific Protocols | |
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WB protocol for Collagen Type I antibody 66761-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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