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验证数据展示

  • WB analysis of mouse skin using 22734-1-AP

    WB analysis of mouse skin using 22734-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 22734-1-AP

    IHC staining of human hepatocirrhosis using 22734-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 22734-1-AP

    IHC staining of human hepatocirrhosis using 22734-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IP experiment of mouse skin using 22734-1-AP

    IP experiment of mouse skin using 22734-1-AP

    IP result of anti-Collagen Type III (N-terminal) (IP:22734-1-AP, 4ug; Detection:22734-1-AP 1:500) with mouse skin tissue lysate 3200ug.

  • IF Staining of human colon using 22734-1-AP

    IF Staining of human colon using 22734-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red).

  • IHC staining of mouse colon using 22734-1-AP

    IHC staining of mouse colon using 22734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of mouse colon using 22734-1-AP

    IHC staining of mouse colon using 22734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IF Staining of mouse colon using 22734-1-AP

    IF Staining of mouse colon using 22734-1-AP

    Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

  • Featured Product
  • KD/KO验证

Collagen Type III (N-terminal) Polyclonal antibody

Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA
Cat No. 22734-1-AP

产品说明书

引用文献(503)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF-P, IF-Fro, IP, ELISA and More (1)

别名

COL3A1, COL3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
HRP
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, mouse kidney tissue, human colon tissue, human skin cancer tissue, mouse heart tissue, mouse colon tissue, human skin tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon tissue
Positive IF-Fro detected inmouse colon tissue
Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22734-1-AP targets Collagen Type III (N-terminal) in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, canine, chicken, bovine, hamster
免疫原 Collagen Type III (N-terminal) fusion protein Ag18658 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type III, alpha 1
别名 COL3A1, COL3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain
计算分子量 1466 aa, 139 kDa
观测分子量 140-180 kDa
GenBank蛋白编号BC028178
基因名称 COL3A1
Gene ID (NCBI) 1281
RRIDAB_2879158
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02461
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

实验方案

Product Specific Protocols
WB protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
IF protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
IP protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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