验证数据展示

IF Staining of mouse skeletal muscle tissue using 17023-1-AP
IF result of anti-COL6A1 (17023-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.
IHC staining of human colon using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COL6A1 antibody included
IHC staining of human colon using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COL6A1 antibody included
WB analysis using 17023-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 17023-1-AP
WB result of Collagen Type VI antibody (17023-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Collagen Type VI transfected HEK-293T cells.
IP experiment of mouse heart using 17023-1-AP
IP result of anti-Collagen Type VI (IP:17023-1-AP, 4ug; Detection:17023-1-AP 1:2000) with mouse heart tissue lysate 1050 ug.
IHC staining of human colon using 17023-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COL6A1 antibody included
IF Staining of human colon using 17023-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon using 17023-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skeletal muscle using 17023-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

COL6A1 Polyclonal antibody

COL6A1 Polyclonal Antibody for WB, IHC, IF, IF-P, IP, ELISA

Cat No. 17023-1-AP

产品说明书

引用文献(35)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF, IF-P, IP, ELISA

别名

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (35)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse heart tissue, HEK-293T cells, human heart tissue, human skeletal muscle tissue, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human colon tissue, human liver tissue, mouse liver tissue, human heart tissue, human hepatocirrhosis tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human colon tissue, mouse skeletal muscle tissue
Positive IF detected in	mouse skeletal muscle tissue

Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 22 publications below
IHC	See 9 publications below
IF	See 9 publications below

产品信息

17023-1-AP targets COL6A1 in WB, IHC, IF, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, zebrafish, sheep
免疫原	COL6A1 fusion protein Ag10288 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	collagen, type VI, alpha 1
别名	COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI
计算分子量	1028 aa, 109 kDa
观测分子量	140 kDa
GenBank蛋白编号	BC052575
基因名称	COL6A1
Gene ID (NCBI)	1291
RRID	AB_2229737
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P12109
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
WB protocol for COL6A1 antibody 17023-1-AP	Download protocol
IHC protocol for COL6A1 antibody 17023-1-AP	Download protocol
IF protocol for COL6A1 antibody 17023-1-AP	Download protocol
IP protocol for COL6A1 antibody 17023-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse,rat	IF	Cell Death Differ Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation Authors - Guangjin Chen View Article
zebrafish	WB,IF	Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis Authors - Ying Liu View Article
human,mouse	WB,IF	Cell Rep The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis Authors - Jingwei Jiang View Article
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human	WB,IHC	Oncogene Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI). Authors - I García-Palmero View Article
human	WB,IHC	Cancer Gene Ther Schwann cell derived pleiotrophin stimulates fibroblast for proliferation and excessive collagen deposition in plexiform neurofibroma Authors - Zhuowei Tian View Article

验证数据展示

IF Staining of mouse skeletal muscle tissue using 17023-1-AP

IHC staining of human colon using 17023-1-AP

IHC staining of human colon using 17023-1-AP

WB analysis using 17023-1-AP

WB analysis of HEK-293T using 17023-1-AP

IP experiment of mouse heart using 17023-1-AP

IHC staining of human colon using 17023-1-AP

IF Staining of human colon using 17023-1-AP

IF Staining of human colon using 17023-1-AP

IF Staining of mouse skeletal muscle using 17023-1-AP

COL6A1 Polyclonal antibody

COL6A1 Polyclonal Antibody for WB, IHC, IF, IF-P, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation

Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis

The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).

Schwann cell derived pleiotrophin stimulates fibroblast for proliferation and excessive collagen deposition in plexiform neurofibroma