验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue, Jurkat cells, Y79 cells, SH-SY5Y cells, HeLa cells, rat thymus tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human tonsillitis tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20882-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | CUL4B fusion protein Ag15024 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cullin 4B |
| Synonyms | KIAA0695, Cullin-4B, cullin 4B |
| Calculated Molecular Weight | 913 aa, 104 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC036216 |
| Gene Symbol | CUL4B |
| Gene ID (NCBI) | 8450 |
| RRID | AB_10694831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13620 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CUL4B antibody 20882-1-AP | Download protocol |
| IHC protocol for CUL4B antibody 20882-1-AP | Download protocol |
| IF protocol for CUL4B antibody 20882-1-AP | Download protocol |
| IP protocol for CUL4B antibody 20882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuropsychopharmacology Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.
| ||
Front Immunol PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway. | ||
Cells Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration, Proliferation and Blood-Testis Barrier Homeostasis. |
