验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, mouse liver tissue, mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18227-1-AP targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 | CYP3A4 fusion protein Ag13074 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 3, subfamily A, polypeptide 4 |
| 别名 | Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A1, CYP3A11, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase |
| 计算分子量 | 503 aa, 57 kDa |
| 观测分子量 | 51-57 kDa |
| GenBank蛋白编号 | BC101631 |
| 基因名称 | CYP3A4 |
| Gene ID (NCBI) | 1576 |
| RRID | AB_2090329 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08684 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984). Human CYP3A4 is homologous to five mouse P450 isoforms, namely, Cyp3a11, Cyp3a16, Cyp3a41a, Cyp3a41b, and Cyp3a44 (PMID: 22446519). And CYP3A4 is also the human homologue of rat CYP3A1 and CYP3A2 (PMID: 27546062, PMID: 32874920).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP3A4 antibody 18227-1-AP | Download protocol |
| IHC protocol for CYP3A4 antibody 18227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions | ||
Hepatology Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening. | ||
Acta Biomater Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply. | ||
ACS Appl Mater Interfaces Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip | ||
Int J Biol Sci Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates | ||
Theranostics Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening. |
