p150 glued Polyclonal antibody
p150 glued Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine and More (1)
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, Jurkat cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
55182-1-AP targets p150 glued in WB, IF, IHC, ELISA applications and shows reactivity with human, Canine samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, Canine |
| Cited Reactivity | human, mussel |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dynactin 1 (p150, glued homolog, Drosophila) |
| Synonyms | DAP 150, DCTN1, DP 150, Dynactin subunit 1, HMN7B, P135, p150 glued |
| Calculated Molecular Weight | 142 kDa |
| Observed Molecular Weight | 135 kDa, 150 kDa |
| GenBank Accession Number | NM_004082 |
| Gene Symbol | DCTN1 |
| Gene ID (NCBI) | 1639 |
| RRID | AB_10837074 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14203 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p150 glued antibody 55182-1-AP | Download protocol |
| IHC protocol for p150 glued antibody 55182-1-AP | Download protocol |
| IF protocol for p150 glued antibody 55182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens. | ||
