验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, PC-3 cells, HEK-293T cells, A431 cells |
| Positive IP detected in | PC-3 cells, A431 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13159-1-AP targets DHX36 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, CoIP, RIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | DHX36 fusion protein Ag3799 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DEAH (Asp-Glu-Ala-His) box polypeptide 36 |
| Synonyms | G4R1, EC:3.6.4.12, DEAH-box protein 36, DEAD/H box polypeptide 36, DDX36 |
| Calculated Molecular Weight | 1008 aa, 115 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC036035 |
| Gene Symbol | DHX36 |
| Gene ID (NCBI) | 170506 |
| RRID | AB_2092157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHX36 antibody 13159-1-AP | Download protocol |
| IHC protocol for DHX36 antibody 13159-1-AP | Download protocol |
| IP protocol for DHX36 antibody 13159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome. | ||
Nat Commun Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs. | ||
Nat Commun Translational control by DHX36 binding to 5'UTR G-quadruplex is essential for muscle stem-cell regenerative functions.
| ||
Nucleic Acids Res A 5' UTR GGN repeat controls localisation and translation of a potassium leak channel mRNA through G-quadruplex formation. | ||
EMBO Rep SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase. |
