E-cadherin Antibody 0 Publications

Mouse Monoclonal| Catalog number: 60335-1-Ig |CloneNo.: 6B11F11

Featured Product KD/KO validated

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Species specificity:
human, pig, mouse

Positive WB detected in:
A431 cells, PC-3 cells; pig brain tissue; A431 cells ; MCF-7 cells

Positive IHC detected in:
human breast cancer tissue, mouse skin tissue

Positive IF detected in:
human breast cancer tissue

Recommended dilution:
WB : 1:2000-1:16000
IHC : 1:1000-1:4000
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cadherin 1, type 1, E-cadherin (epithelial)

Calculated molecular weight:
882aa,97 kDa

Observed molecular weight:
120 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CDH1

Synonyms
Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin
Background

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.


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