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验证数据展示

  • WB analysis of HEK-293 using 11172-1-AP

    WB analysis of HEK-293 using 11172-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11172-1-AP

    IHC staining of human breast cancer using 11172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11172-1-AP

    IHC staining of human breast cancer using 11172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11172-1-AP

    IF Staining of HepG2 using 11172-1-AP

    Immunofluorescent analysis of HepG2 cells using 11172-1-AP (EAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EAF2 Polyclonal antibody

EAF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11172-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

TRAITS, Testosterone-regulated apoptosis inducer and tumor suppressor protein, ELL-associated factor 2, ELL associated factor 2, BM-040

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11172-1-AP targets EAF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 EAF2 fusion protein Ag1637 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ELL associated factor 2
别名 TRAITS, Testosterone-regulated apoptosis inducer and tumor suppressor protein, ELL-associated factor 2, ELL associated factor 2, BM-040
计算分子量 42 kDa
观测分子量 45 kDa
GenBank蛋白编号BC014209
基因名称 EAF2
Gene ID (NCBI) 55840
RRIDAB_2097560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#11172-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF2.

实验方案

Product Specific Protocols
WB protocol for EAF2 antibody 11172-1-APDownload protocol
IHC protocol for EAF2 antibody 11172-1-APDownload protocol
IF protocol for EAF2 antibody 11172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao
View Article
humanWB,IHC

Biol Proced Online

EAF2 Downregulation Recruits Tumor-associated Macrophages in Prostate Cancer through Upregulation of MIF

Authors - Tianyu Cao
  • KD Validated
View Article