验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse kidney tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11172-1-AP targets EAF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | EAF2 fusion protein Ag1637 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ELL associated factor 2 |
Synonyms | TRAITS, Testosterone-regulated apoptosis inducer and tumor suppressor protein, ELL-associated factor 2, ELL associated factor 2, BM-040 |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC014209 |
Gene Symbol | EAF2 |
Gene ID (NCBI) | 55840 |
RRID | AB_2097560 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96CJ1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#11172-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF2.
实验方案
Product Specific Protocols | |
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WB protocol for EAF2 antibody 11172-1-AP | Download protocol |
IHC protocol for EAF2 antibody 11172-1-AP | Download protocol |
IF protocol for EAF2 antibody 11172-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Biol Proced Online EAF2 Downregulation Recruits Tumor-associated Macrophages in Prostate Cancer through Upregulation of MIF
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