验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, A431 cells, HeLa/RPE1 cells, NCI-H1299 cells, HeLa cells, A549 cells, L02 cells, NCL-H1299 cells |
| Positive IP detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18986-1-AP targets EGFR-Specific in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, ChIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, sheep |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Synonyms | EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1 |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 145-165 kDa |
| GenBank Accession Number | NM_005228 |
| Gene Symbol | EGFR |
| Gene ID (NCBI) | 1956 |
| RRID | AB_10596476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EGFR-Specific antibody 18986-1-AP | Download protocol |
| IP protocol for EGFR-Specific antibody 18986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells | ||
Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.
| ||
Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma | ||
Biosens Bioelectron Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic. | ||
Cancer Res Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner. |
