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验证数据展示

  • WB analysis of MCF-7 using 11193-1-AP

    WB analysis of MCF-7 using 11193-1-AP

    WB result of EIF1AY antibody (11193-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF1AY transfected MCF-7 cells.

  • WB analysis of MCF-7 using 11193-1-AP

    WB analysis of MCF-7 using 11193-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11193-1-AP (EIF1AY antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 11193-1-AP

    IF Staining of MCF-7 using 11193-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11193-1-AP (EIF1AY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

EIF1AY Polyclonal antibody

EIF1AY Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11193-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-4C, eIF-1A Y isoform, eIF1A Y isoform, eIF 4C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11193-1-AP targets EIF1AY in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen EIF1AY fusion protein Ag1680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 1A, Y-linked
Synonyms Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-4C, eIF-1A Y isoform, eIF1A Y isoform, eIF 4C
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC005248
Gene Symbol EIF1AY
Gene ID (NCBI) 9086
RRIDAB_2262159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF1AY is 1 of 5 NRY genes that are expressed in many tissues and have homologs on the X chromosome, which is an essential translation initiation factor. EIF1AY seems to be required for maximal rate of protein biosynthesis, and enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. This is a rabbit polyclonal antibody raised against the full length of human EIF1AY, and can recognize both EIF1AY and EIF1AX.

实验方案

Product Specific Protocols
WB protocol for EIF1AY antibody 11193-1-APDownload protocol
IF protocol for EIF1AY antibody 11193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols