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NSE Polyclonal antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10149-1-AP

Featured Product KD/KO validated

浓度: 400 μg/ml

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Applications

Tested Applications:
IHC, IP, WB,ELISA
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Cited Applications:
IF, IHC, WB


Images See all

WB analysis of human brain using 10149-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

WB analysis of U-251 using 10149-1-AP

WB result of NSE antibody (10149-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells.

IP experiment of mouse brain using 10149-1-AP

IP Result of anti-NSE (IP:10149-1-AP, 4ug; Detection:10149-1-AP 1:300) with mouse brain tissue lysate 4000ug.

IHC staining of human brain using 10149-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10149-1-AP (NSE antibody at dilution of 1:200 (under 10x lens).

IHC staining of human brain using 10149-1-AP

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10149-1-AP (NSE antibody at dilution of 1:200 (under 40x lens).

Species Specificity:
human, mouse, rat
Cited Species:
goat, human, rat
Positive WB detected in:
human brain tissue, mouse brain tissue, U-251 cells
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human brain tissue, human lung tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:5000-1:10000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NSE fusion protein Ag0196
Full name:
enolase 2 (gamma, neuronal)
Calculated molecular weight:
47 kDa
Observed molecular weight:
47 kDa
GenBank accession number:
BC002745
Gene ID (NCBI):
2026
Gene symbol
ENO2
Synonyms
enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

Background

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

Protocols

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