验证数据展示

WB analysis of A431 using 21050-1-AP
WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.
WB analysis using 21050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 21050-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with EPCAM/CD326 antibody included
IHC staining of human colon cancer using 21050-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with EPCAM/CD326 antibody included
IF Staining of MCF-7 using 21050-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EPCAM antibody (21050-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 21050-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 21050-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 21050-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HT-29 using 21050-1-AP
1x10^6 HT-29 cells were surface stained with 0.5 ug EPCAM/CD326 Polyclonal antibody (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.5 ug Control Antibody (blue). Cells were not fixed.
IF Staining of HT-29 using 21050-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA

Cat No. 21050-1-AP

Print datasheet

Publications(78)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA and More (2)

Synonyms

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (78)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	A431 cells, mouse colon tissue, rat colon tissue
Positive IHC detected in	human colon cancer tissue, mouse colon tissue, human breast cancer tissue, human colon tissue, mouse stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse colon tissue, MCF-7 cells, mouse lung tissue
Positive IF-Fro detected in	mouse colon tissue
Positive IF/ICC detected in	HT-29 cells, MCF-7 cells
Positive FC detected in	HT-29 cells

Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:500-1:2000
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC)	FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 41 publications below
IHC	See 14 publications below
IF	See 28 publications below
FC	See 2 publications below
ChIP	See 1 publications below

产品信息

21050-1-AP targets EPCAM/CD326 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA Application Description
Cited Applications	WB, IHC, IF, FC, chIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Immunogen	EPCAM/CD326 fusion protein Ag15393 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	epithelial cell adhesion molecule
Synonyms	EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1
Calculated Molecular Weight	314 aa, 35 kDa
Observed Molecular Weight	35-40 kDa
GenBank Accession Number	BC014785
Gene Symbol	EPCAM
Gene ID (NCBI)	4072
RRID	AB_10693684
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P16422
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 21050-1-AP	Download protocol
IHC protocol for EPCAM/CD326 antibody 21050-1-AP	Download protocol
IF protocol for EPCAM/CD326 antibody 21050-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma Authors - Han Fang View Article
mouse	IHC	Cancer Cell Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression. Authors - Chunxiao Xu View Article
mouse	IF	Gut Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis. Authors - Yunben Yang View Article
human	IF	Nat Commun Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma. Authors - Zhaohui Chen View Article
human	WB	Biosens Bioelectron Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier. Authors - Hao Lu View Article
human	WB	EMBO J A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. Authors - Doowon Huh View Article

验证数据展示

WB analysis of A431 using 21050-1-AP

WB analysis using 21050-1-AP

IHC staining of human colon cancer using 21050-1-AP

IHC staining of human colon cancer using 21050-1-AP

IF Staining of MCF-7 using 21050-1-AP

IF Staining of mouse colon using 21050-1-AP

IF Staining of mouse colon using 21050-1-AP

IF Staining of mouse colon using 21050-1-AP

FC experiment of HT-29 using 21050-1-AP

IF Staining of HT-29 using 21050-1-AP

EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma

Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.

Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.

A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.