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验证数据展示

  • WB analysis using 24344-1-AP

    WB analysis using 24344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24344-1-AP (ERP29 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24344-1-AP

    IF Staining of HeLa using 24344-1-AP

    Immunofluorescent analysis of HeLa cells using 24344-1-AP (ERP29 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human liver cancer using 24344-1-AP

    IHC staining of human liver cancer using 24344-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 24344-1-AP

    IHC staining of human liver cancer using 24344-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24344-1-AP (ERP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse liver using 24344-1-AP

    IP experiment of mouse liver using 24344-1-AP

    IP result of anti-ERP29 (IP:24344-1-AP, 4ug; Detection:24344-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • IF Staining of human liver cancer using 24344-1-AP

    IF Staining of human liver cancer using 24344-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human liver cancer using 24344-1-AP

    IF Staining of human liver cancer using 24344-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ERP29 antibody (24344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ERP29 Polyclonal antibody

ERP29 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 24344-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

Synonyms

PDIA9, PDI DB, ERp31, ERp28, ERP 29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue, mouse lung tissue, rat testis tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24344-1-AP targets ERP29 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ERP29 fusion protein Ag19451 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endoplasmic reticulum protein 29
Synonyms PDIA9, PDI DB, ERp31, ERp28, ERP 29
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC101495
Gene Symbol ERP29
Gene ID (NCBI) 10961
RRIDAB_2879503
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).

实验方案

Product Specific Protocols
WB protocol for ERP29 antibody 24344-1-APDownload protocol
IHC protocol for ERP29 antibody 24344-1-APDownload protocol
IF protocol for ERP29 antibody 24344-1-APDownload protocol
IP protocol for ERP29 antibody 24344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

iScience

Non-canonical function of DPP4 promotes cognitive impairment through ERp29-associated mitochondrial calcium overload in diabetes

Authors - Jiaxiu Li
View Article
mouseWB,IF,CoIP

Metabolism

Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization

Authors - Ya Hui
View Article