验证数据展示
经过测试的应用
| Positive WB detected in | mouse small intestine tissue, HepG2 cells |
| Positive IHC detected in | human small intestine tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13781-1-AP targets FABP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | FABP6 fusion protein Ag4788 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fatty acid binding protein 6, ileal |
| 别名 | I-BABP, I-15P, GT, Gastrotropin, Fatty acid-binding protein 6 |
| 计算分子量 | 177 aa, 20 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC022489 |
| 基因名称 | FABP6 |
| Gene ID (NCBI) | 2172 |
| RRID | AB_2877976 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51161 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fatty acid binding protein 6 (FABP6, also known as the ileal bile acid binding protein IBABP) is regarded as a bile acid binding protein found in the distal portion of the small intestine and may be important in maintaining bile acid homeostasis(PMID: 25754072). FABP6 is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer(PMID: 17909007). And also, it was showed a potential drug target for the treatment of diabetes(PMID: 27500412). There are 2 isoforms of this protein,one of which is about 14 kDa we detected.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FABP6 antibody 13781-1-AP | Download protocol |
| IHC protocol for FABP6 antibody 13781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
Biomaterials MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice. | ||
J Agric Food Chem Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice. | ||
J Immunol Res FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells
| ||
Int Immunopharmacol Melatonin alleviates necrotizing enterocolitis by reducing bile acid levels through the SIRT1/FXR signalling axis |