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FBXW7 Polyclonal antibody

FBXW7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 55290-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA

AGO, Archipelago homolog, CDC4, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, hAgo, hCdc4, SEL 10, SEL10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman skin cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FBXW7 Ready-To-Use IHC Kit. FBXW7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55290-1-AP targets FBXW7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and WD repeat domain containing 7
别名 AGO, Archipelago homolog, CDC4, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, hAgo, hCdc4, SEL 10, SEL10
计算分子量 80 kDa
观测分子量100-110 kDa, 66-75 kDa
GenBank蛋白编号NM_018315
基因名称 FBXW7
Gene ID (NCBI) 55294
RRIDAB_2881300
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969H0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.

实验方案

Product Specific Protocols
WB protocol for FBXW7 antibody 55290-1-APDownload protocol
IHC protocol for FBXW7 antibody 55290-1-APDownload protocol
IF protocol for FBXW7 antibody 55290-1-APDownload protocol
IP protocol for FBXW7 antibody 55290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication

Authors - Jingkun Zeng
  • KD Validated
humanWB

iScience

Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal

Authors - Da-Wei Yeh
humanWB

Oncotarget

PMID: 28404959

Authors - Min Zheng
mouseWB

Endocrinology

Rictor regulates spermatogenesis by controlling Sertoli cell cytoskeletal organization and cell polarity in the mouse testis.

Authors - Heling Dong
humanWB

Int J Biochem Cell Biol

The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc.

Authors - Zhijun Xu
humanWB

Biochim Biophys Acta Gen Subj

FBXO8 is a novel prognostic biomarker in different molecular subtypes of breast cancer and suppresses breast cancer progression by targeting c-MYC

Authors - Abdul Jamil Khan
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