• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FCGR2B / CD32b Polyclonal antibody

FCGR2B / CD32b Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21541-1-AP

Print datasheet

Synonyms

FCGR2B, Fc-gamma RII-b, FCG2, Fc gamma RIIb, Fc gamma RII b

验证数据展示

  • WB analysis of human placenta using 21541-1-AP

    WB analysis of human placenta using 21541-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21541-1-AP

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2B antibody included
  • IHC staining of human tonsillitis using 21541-1-AP

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2B antibody included

经过测试的应用

Positive WB detected inhuman placenta tissue, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FCGR2B Ready-To-Use IHC Kit. FCGR2B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21541-1-AP targets FCGR2B / CD32b in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FCGR2B / CD32b fusion protein Ag16189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIb, receptor (CD32)
Synonyms FCGR2B, Fc-gamma RII-b, FCG2, Fc gamma RIIb, Fc gamma RII b
Calculated Molecular Weight 310 aa, 34 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC031992
Gene Symbol CD32b
Gene ID (NCBI) 2213
RRIDAB_2878881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31994
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
IHC protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Ther

High-dose systemic adeno-associated virus vector administration causes liver and sinusoidal endothelial cell injury

Authors - Juliette Hordeaux
View Article