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验证数据展示

  • WB analysis of NIH/3T3 using 18073-1-AP

    WB analysis of NIH/3T3 using 18073-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18073-1-AP (FKBP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 18073-1-AP

    IF Staining of L02 using 18073-1-AP

    Immunofluorescent analysis of L02 cells using 18073-1-AP (FKBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FKBP9 Polyclonal antibody

FKBP9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18073-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FK506-binding protein 9, FK506 binding protein 9, EC:5.2.1.8, 63 kDa FKBP, 63 kDa FK506-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, L02 cells
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18073-1-AP targets FKBP9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FKBP9 fusion protein Ag12715 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein 9, 63 kDa
Synonyms FK506-binding protein 9, FK506 binding protein 9, EC:5.2.1.8, 63 kDa FKBP, 63 kDa FK506-binding protein
Calculated Molecular Weight 570 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC101723
Gene Symbol FKBP9
Gene ID (NCBI) 11328
RRIDAB_2878490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95302
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP9 (FK506-binding protein 9, also referred to as FKBP60 or FKBP63) belongs to a family of immunophilins that are able to bind to FK506, an immunosuppressive drug. Several members of the FKBP family, including FKBP9, FKBP13, FKBP23 and FKBP65, localize to the endoplasmic reticulum (ER) as they contain the ER retention motif H/R/KDEL (PMID:32111229). In addition, the FKBP9 mutation was also reported to be associated with disease-free survival rates in pheochromocytoma or paraganglioma (PMID:28386672).

实验方案

Product Specific Protocols
WB protocol for FKBP9 antibody 18073-1-APDownload protocol
IF protocol for FKBP9 antibody 18073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genes Dis

The stability of FKBP9 maintained by BiP is crucial for glioma progression

Authors - Shirong Li
View Article