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Fibronectin Polyclonal antibody
Fibronectin Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
657
验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, NIH/3T3 cells, MDA-MB-453s cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15613-1-AP targets Fibronectin in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, ELISA |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, bovine, hamster, sheep, medaka embryos |
| Immunogen | Fibronectin fusion protein Ag8004 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fibronectin 1 |
| Synonyms | FN1, fibronectin 1, ED B, Cold-insoluble globulin, Cold insoluble globulin |
| Calculated Molecular Weight | 2386 aa, 263 kDa |
| Observed Molecular Weight | 250-275 kDa |
| GenBank Accession Number | BC005858 |
| Gene Symbol | Fibronectin |
| Gene ID (NCBI) | 2335 |
| RRID | AB_2105691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02751 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibronectins play a role in cell adhesion, motility, wound healing, and the maintenance of cell shape. Fibronectins bind to cell surfaces via interactions with integrins and various molecules such as collagen, fibrin, heparin, DNA, and actin (PMID: 3326130). Cellular interaction with fibronectin (FN1) promotes cell cycle progression and proliferation by influencing cyclins.
What is the molecular weight of FN1?
The molecular weight of FN1 is 220 kDa.
What is the tissue specificity of FN1?
FN1 is a large glycoprotein that exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1) (PMID: 21923916). Plasma FN1, or the dimeric form, is secreted by hepatocytes, whereas cellular FN1, the dimeric or cross-linked multimeric form, is produced by fibroblasts and epithelial cells and deposited as fibrils in the extracellular matrix.
What are the post-translational modifications of FN1?
FN1 is often sulfated and its C-terminal NC1 peptide, anastellin, is produced as a result of proteolytic processing and can inhibit tumor growth, angiogenesis, and metastasis by activating p38 MAPK and inhibiting lysophospholipid signaling (PMID: 11209058).
What is FN1's role in embryogenesis?
Fibronectin has a crucial role in the development of the left-right body axis during embryogenesis (PMID: 21466802).
What is FN1's involvement in disease?
Fibronectin glomerulopathy is a kidney disease that eventually leads to end-stage renal disease and is caused by mutations in the FN1 gene. Mutations in FN1 lead to the production of abnormal fibronectin protein that is deposited in the glomeruli of the kidney (PMID: 18268355).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibronectin antibody 15613-1-AP | Download protocol |
| IHC protocol for Fibronectin antibody 15613-1-AP | Download protocol |
| IF protocol for Fibronectin antibody 15613-1-AP | Download protocol |
| IP protocol for Fibronectin antibody 15613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine | ||
Cell Metab The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity. | ||
J Clin Invest Mitochondrial dysfunction in macrophages promotes inflammation and suppresses repair after myocardial infarction | ||
Nat Protoc Responsive and activable nanomedicines for remodeling the tumor microenvironment. | ||
Nat Commun Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment | ||
Acta Pharm Sin B KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer |
