GAC-specific Polyclonal antibody

GAC-specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 19958-1-AP

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Synonyms

AAD20, GAC, GAC-specific, GLS, GLS1, glutaminase C, K glutaminase, KIAA0838, L glutamine amidohydrolase



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19958-1-AP targets GAC-specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutaminase
Synonyms AAD20, GAC, GAC-specific, GLS, GLS1, glutaminase C, K glutaminase, KIAA0838, L glutamine amidohydrolase
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberAF158555
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_10640899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.

实验方案

Product Specific Protocols
WB protocol for GAC-specific antibody 19958-1-APDownload protocol
IHC protocol for GAC-specific antibody 19958-1-APDownload protocol
IP protocol for GAC-specific antibody 19958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection.

Authors - Maria L Balmer
mouseIF

Cell Stem Cell

Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism.

Authors - Pia Sommerkamp
humanWB,IP

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
humanWB

Blood

Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Authors - Nathalie Jacque
humanWB

Oncotarget

Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.

Authors - Yue-Zhi Lee
ratCoIP, WB

Int J Mol Sci

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Authors - Małgorzata Beręsewicz-Haller